Journal article
DNP NMR spectroscopy reveals new structures, residues and interactions in wild spider silks
HC Craig, SJ Blamires, MA Sani, MM Kasumovic, A Rawal, JM Hook
Chemical Communications | ROYAL SOC CHEMISTRY | Published : 2019
DOI: 10.1039/C9CC01045A
Abstract
DNP solid state NMR spectroscopy allows non-targeted analysis of wild spider silk in unprecedented detail at natural abundance, revealing hitherto unreported features across several species. A >50-fold signal enhancement for each silk, enables the detection of novel H-bonding networks and arginine conformations, and the post-translational modified amino acid, hydroxyproline.
Related Projects (2)
Grants
Awarded by Australian Research Council
Funding Acknowledgements
We acknowledge a Hermon Slade Foundation grant to SB and AR (HSF 17/6); Australian Research Council grants to MMK for a Future Fellowship, FT140100115 (2014-2019), and to MAS for the equipment LE160100120. Aspects of this research were facilitated by access to the Australian Proteome Analysis Facility supported under the Australian Government's National Collaborative Research Infrastructure Strategy (NCRIS). Access to the DNP-NMR spectrometer was supported through collaboration with the Bio21 Institute, Melbourne University, Australia and financial assistance from the Mark Wainwright Analytical Centre, University of NSW, Sydney, Australia.